Genomic, epigenomic, and biophysical cues controlling the emergence of the lung alveolus
Updated October 9, 2023All mouse experiments used CD-1 female mice with the age indicated in the libraries . Single-cell suspensions were prepared from whole mouse lungs. For all libraries, DAPI-negative cells were were sorted by flow cytometry to obtain heterogeneous whole lung sample. Human tissue was derived from normal peripheral lung tissue. Single cell suspension was depleted of CD45-positive cells by bead selection. All of the murine post-natal timepoints also had CD45-positive cells removed from cell sort. Single-cell barcoded droplets were produced using 10X Single Cell 3’ v2 chemistry. Libraries generated were sequenced using HiSeq2500 instrument in High-output mode. Reads were aligned and gene level unique molecular identifier (UMI) counts were obtained using the Cell Ranger pipeline.
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Analysis Portals
Project Label
Genomic, epigenomic, and biophysical cues controlling the emergence of the lung alveolusSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
trueAnalysis Protocol
1c145725-c287-45c8-b0d7-6b5ed78674fc, 823cb2ce-79ff-4737-82d8-c50c286ede54File Format
Cell Count Estimate
UnspecifiedDonor Count
7