CITE-Seq of COVID Lungs

The LungMAP program has been working for over a decade to provide the lung community with a comprehensive resource for the lung research community, including reference data sets and protocols. The University of Rochester has served as the Human Tissue Core and worked extensively with Research Center lab groups. We have established protocols for procuring human tissue including, but not limited to lung, and processing them for a variety of downstream analysis modalities including the generation of single cell suspensions. We have successfully utilized singe cells suspensions to generate single cell CITE-Seq data utilizing the 10X Genomics Single Cell 3' (v3.1) RNA sequencing platform. This protocol describes the steps necessary for preparing generating sequencing data following staining single cells with ADT-tagged antibodies (including library preparation, sequencing steps, and mapping steps).

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